The effectiveness of some antibiotic and chemical compounds for controlling bacterial wilt caused by Ralstonia solanacearum from marigold (Tagetes papula L.)

Ngoc Tam Huynh1,2, Lan Phuong To3, Uyen Thanh Le1,4,
1 Post-graduate, Can Tho University, Vietnam
2 Tram Chim high school, Dong Thap province, Vietnam
3 Le Vu Hung Learning Resource Center, Dong Thap University, Vietnam
4 Faculty of Natural Sciences Teacher Education, Dong Thap University, Vietnam

Main Article Content

Abstract

The effectiveness of three antibiotic and chemical compounds in controlling wilt disease caused by Ralstonia solanacearum from Tagetes papula L. in vitro and in net-house conditions are evaluated. In vitro, these compounds were tested on 10 strains of R. solanacearum to find bacterial inhibit effect at 72 hours after inoculation. From there, the compound of Streptomycin + Oxytetracycline is higher effect in inhibitting R. solanacearum strains than the other two compounds as Oxytetracyline hydrochloride + Gentamicin sulphate, or Oxolinic acid. Under net-house condition, the compound of Streptomycin + Oxytetracycline and two strains of R. solanacearum RM3 and RM4 with the least sensitive characteristic to this compound are tested to evaluating the effectiveness of disease controlling on marigold plants. Through 6, 10, 14, 18 days after infection, both treatments infected with separate strains of RM3 or RM4 after applied the Streptomycin + Oxytetracycline compound have showed satisfactory effect. The effectiveness of reducing bacterial wilt disease is high with ranging from 46.7% to 57.2%. At the same time, when the results of appling are compared with the treatments that only infected with RM3 or RM4, their AUDPC index are also decreased. In addition, their severity index are reduced while they compared with treatments that only infecting with RM3 or RM4. The compound of Streptomycin + Oxytetracycline has shown effective disease reduction in the prevention and treatment of bacterial wilt caused by R. solanacearum on marigold, and it can be selected as one of the preventive measures this disease.

Article Details

References

Abbott, W. S. (1925). A method for computing the effectiveness of an insecticide. Journal of econmic entomology, 18, 265-269.
Ateka, E. M., Mwang'Ombe A. W., & Kimenju J. W. (2001). Reaction of Potato Cultivars to Ralstonia solanacearum in Kenya. African Crop Science Journal., 9(1), 251-256.
Bộ Nông nghiệp và Phát triển nông thôn. (2020). Thông tư ban hành danh mục thuốc bảo vệ thực vật được phép sử dụng, cấm sử dụng tại Việt Nam. Hà Nội: Thông tư của Bộ Nông nghiệp và Phát triển nông thôn.
Huỳnh, N. T., Lê, U. T., Trần, T. T., Nguyễn, M. Đ., Lưu, T. D., & Nguyễn, T. T. N. (2019). Đánh giá hiệu quả phòng trị của thực khuẩn thể và thuốc trừ vi khuẩn đối với bệnh héo xanh do vi khuẩn Ralstonia solanacearum trên cây hoa cúc (Chrysanthemum sp.) ở điều kiện ngoài đồng. Tạp chí Nông nghiệp và Phát triển nông thôn, Chuyên đề Công nghệ sinh học trong nông nghiệp, 95-100.
Jeger, M. J., & Viljanen-Rollinson, S. L. H. (2001). The use of the area under the disease-progress curve (AUDPC) to assess quantitative disease resistance in crop cultivars. Theoritical and Applied Genetic, 102(1), 32-40.
Maia, P. P., Silva, E. C., Rath, S., & Reyes, F. G. R. (2009). Residue content of oxytetracycline applied on tomatoes grown in open field and greenhouse. Food Control, 20, 11-16. http://dx.doi.org/10.1016/j.foodcont.2008.01.007
Nguyễn, T. T. C., & Trần, T. T. T. (2014). Dịch hại trên hoa hồng, Cúc, mai, Vạn thọ. Cần Thơ: NXB Đại học Cần Thơ.
Shtienberg, D., Zilberstaine, M., Oppenheim, D., Herzog, Z., Manulis, S., Shwartz, H., & Kritzman, G. (2001). Efficacy of Oxolinic Acid and Other Bactericides in Suppression of Erwinia amylovora in Pear Orchards in Israel. Phytoparasitica, 29(2), 143-154.
Winstead, N. N., & Kelman, A. (1952). Inoculation techniques for evaluating resistance to Pseudomonas solanacearum. Phytopathology, 42, 628-634.