Investigating antioxidant extraction from Receptaculum Nelumbinis

Thi Hong Nhung Dang1, Dang Khoa Tran1, Duy Khuong Pham1, Hong Tham Nguyen1, Thi Hong Hanh Nguyen2,
1 Student, Faculty of Natural Sciences, Dong Thap University, Vietnam
2 Faculty of Natural Sciences, Dong Thap University, Vietnam

Main Article Content

Abstract

Receptaculum Nelumbinis, also known as Lotus receptacle, is a byproduct of the seed-harvesting process of Nelumbo nucifera. It is dried and used as fuel, aesthetic decoration or for cooking drinking water. Extracts from Lotus receptacle contain several active compounds such as alkaloids, polyphenols, flavonoids, glycosides, tannins, steroids, and saponins. Among these, polyphenols are a compound of strong antioxidant activity. In this study, the extraction method and solvent were investigated to increase the polyphenol content. The polyphenol and flavonoid contents and the antioxidant activity of the Lotus receptacle extract were also evaluated. The results showed that the highest yield of the extract (34,06%) was obtained using the reflux method with 70% ethanol as the solvent, a 90-minute extraction time, and a temperature of 70oC. The chemical composition of the Lotus receptacle extract included polyphenols, alkaloids, flavonoids, terpenoids, tannins, and coumarins. The extract’s flavonoid and polyphenol contents were determined to be 361,86 ± 11,16 mg QE/g and 585,12 ± 6,01 mg GAE/g, respectively. The Lotus receptacle extract also exhibited strong antioxidant activity, as demonstrated by its ability to scavenge DPPH and ABTS•+ free radicals and its reducing power (RP), with corresponding EC50 values of 11,10 ± 0,14 μg/mL, 6,78 ± 0,52 μg/mL and 56,05 ± 0,73 μg/mL, respectively. These results suggest that the Lotus receptacle is a potential source of antioxidant phytochemicals.

Article Details

References

Arooj, M., Imran, S., Inam-ur-Raheem, M., Riaz, M. S., Sameen, A., Siddique, R., Sahar, A., Tariq, S., Riaz, A., Hussain, A., Siddeeg, A., & Aadil, R. (2021). Lotus seeds (Nelumbinis semen) as an emerging therapeutic seed: A comprehensive review. Food Science and Nutrition, 9, 3971-3987. https://doi.org/10.1002/fsn3.2313.
Bag, G. C., Devi, P. G., & Bhaigyabati, T. (2015). Assessment of total flavonoid content and antioxidant activity of methanolic rhizome extract of three Hedychium species of Manipur Valley. International Journal of Pharmaceutical Sciences Review and Research, 30(1), 154-159. https://doi.org/10.4236/vp.2020.64020.
Cao, T. T., & Dương, N. T. (2019). Phân tích hiệu quả sản xuất sen tại huyện Tháp Mười tỉnh Đồng Tháp. Tạp chí Khoa học Đại học Đồng Tháp, 39, 26-34. https://doi.org/10.52714/dthu.39.8.2019.713.
Đặng, K. T., & Nguyễn, T. M. T. (2020). Nghiên cứu khả năng hấp thụ Cu2+ trong mội trường nước của composite polyaniline-gương sen. Tạp chí Khoa học công nghệ Việt Nam, 62(11), 7-11.
Đỗ, T. L. (2004). Những cây thuốc và vị thuốc Việt Nam Hà Nội: NXB Y học.
Gülçin, İ. (2012). Antioxidant activity of food constituents: an overview. Archives of Toxicology, 86(3), 345-391. https://sci-hub.ee/https://doi.org/10.1007/s00204-011-0774-2.
Huỳnh, T. K. (2017). Khảo sát hoạt tính các hợp chất kháng oxy hóa trong lá và thân cây chùm ngây (Moringa oleifera). Tạp chí Khoa học Đại học cần Thơ, (CĐ Nông nghiệp 2016), 179-184. https://doi.org/10.22144/ctu.jsi.2016.086.
Kim, M. J., & Shin, H. S. (2012). Antioxidative effect of lotus seed and seedpod extracts. Food Science and Biotechnology, 21(6), 1761-1766. https://doi.org/10.1007/s10068-012-0234-7.
Liu, Y. T., Lai, Y. H., Lin, H. H., & Chen, J. H. (2019). Lotus Seedpod Extracts Reduced Lipid Accumulation and Lipotoxicity in Hepatocytes. Nutrients, 11(12), 2895. https://doi.org/ 10.3390/nu11122895.
Mensor, L. L., Menezes, F. S., Leitão, G. G., Reis, A. S., Santos, T. C. D., Coube, C. S., & Leitão, S. G. (2001). Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy research : PTR, 15, 127-130. https://doi.org/10.1002/ptr.687.
Nenadis, N., Wang, L. F., Tsimidou, M., & Zhang, H. Y. (2004). Estimation of scavenging activity of phenolic compounds using the ABTS(*+) assay. Journal of Agricultural and Food Chemistry, 52(15), 4669-4674. https://doi.org/10.1021/jf0400056.
Oyaizu, M. (1986). Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese Journal of Nutrition and Dietetics, 44(6), 307-315.
Phạm, H. H. (1999). Cây cỏ Việt Nam (Quyển 3). Thành phố Hồ Chí Minh: NXB Trẻ.
Sharma, O. P., & Bhat, T. K. (2009). DPPH antioxidant assay revisited. Food Chemistry, 113(4), 1202-1205. https://doi.org/10.1016/j.foodchem.2008.08.008.
Shen, Y., Guan, Y., Song, X., He, J., Xie, Z., Zhang, Y., Zhang, H., & Tang, D. (2019). Polyphenols extract from lotus seedpod (Nelumbo nucifera Gaertn.): Phenolic compositions, antioxidant, and antiproliferative activities. Food Science and Nutrition, 7(9), 3062-3070. https://doi.org/10.1002/fsn3.1165.
Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology. 299 (152-178). Elsevier: Academic Press.
Sofowora, A. (1996). Research on medicinal plants and traditional medicine in Africa. Journal of Alternative and Complementary Medicine, 2(3), 365-372.
Tiwari, P. K., Kaur, M., & Kaur, H. (2011). Phytochemical Screening and Extraction: A Review. Internationale Pharmaceutica Sciencia, 1, 98-106.
Wang, Z., Cheng, Y., Zeng, M., Wang, Z., Qin, F., Wang, Y., Chen, J., & He, Z. (2021). Lotus (Nelumbo nucifera Gaertn.) leaf: A narrative review of its Phytoconstituents, health benefits and food industry applications. Trends in Food Science & Technology, 112, 631-650. https://doi.org/10.1016/j.tifs.2021.04.033.