Thanh bên bài viết

PDF
Ngày xuất bản: 10/10/2020
Ngày xuất bản Online: 10/10/2020
Số lượt xem tóm tắt: 49
Số lượt xem PDF: 21
DOI: https://doi.org/10.52714/dthu.9.5.2020.821
Số xuất bản
Tập 9 Số 5 (2020): Chuyên san Khoa học Tự nhiên (Tiếng Anh)
Chuyên mục
Khoa học Tự nhiên (Tiếng Anh)
Trích dẫn bài báo
Ha, D. D., Nguyen, T. O., & Ha, H. H. V. (2020). Acetochlor degradation by a mixed culture of P. fluorescens KT3 and B. subtilis 2M6E immobilized in alginate. Tạp chí Khoa học Đại học Đồng Tháp, 9(5), 86-92. https://doi.org/10.52714/dthu.9.5.2020.821

Acetochlor degradation by a mixed culture of P. fluorescens KT3 and B. subtilis 2M6E immobilized in alginate

Ha Danh Duc1, , Nguyen Thi Oanh2, Ha Huynh Hong Vu1
1 Department of Engineering and Information Technology, Dong Thap University, Vietnam
2 Center for Chemical Analysis, Dong Thap University, Vietnam

Nội dung chính của bài viết

Tóm tắt

In this study, the acetochlor degradation by two microbial isolates, P. fluorescens KT3 and B. subtilis 2M6E, was determined. The immobilization of the bacterial mixture in alginate beads resulted in higher degradation rates compared to their free cells. The addition of glycerol as a cryoprotectant reducing adverse effects in long-term storage. After storing at 4oC for three months, the cell survivals of free cell with and without the cryoprotectant were 43.0 ± 6.1% and 57.3 ± 5.9%, while data for immobilized bacteria were 64.0 ± 5.3% and 77.6 ± 4.0%, respectively. These results prove that the immobilization of bacteria in alginate and the addition of glycerol can be applied for storing bacteria in a long-term period.

Chi tiết bài viết

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Từ khóa

Acetochlor, degradation, P. fluorescens KT3, B. subtilis 2M6E, immobilization

Tài liệu tham khảo

Bai, X., Ye, Z., Li, Y., Yang, L., Qu, Y., & Yang, X. (2010). Preparation and characterization of a novel macroporous immobilized micro- organism carrier. Biochem Eng, (49), 264-270.
De Guzman, N.P., Hendley, P., Gustafson, D.I., van Wesenbeeck I., Klein A.J., Fuhrman, J.D., Travis, K., Simmons, N.D., Teskey, W.E., & Durham, R.B. (2005). The acetochlor registration partnership state ground water monitoring program. J Environ Qual, (34), 793-803.
Duc, H.D., & Oanh, N.T. (2019). Biodegradation of acetochlor and 2-methyl-6-ethylaniline by Bacillus subtilis and Pseudomonas fl uorescens. Microbiology, (88), 729-738.
Garcia, A.M. (2003). Pesticides exposure and women's health. Am J Ind Med, (44), 584-594.
Jablonkai, I. (2000). Microbial and photolytic degradation of the herbicide acetochlor”, Int J Environ Anal Chem, (78), 1-8.
Junghans, M., Backhaus, T., Faust, M., Scholze, M., & Grimme, L.H. (2003). Predictability of combined eff ects of eight chloroacetanilide herbicides on algal reproduction. Pest Manag Sci, (59), 1101-1110.
Kearney, L., Upton, M., & Mc Loughlin, A. (1990). Enhancing the viability of Lactobacillus plantarum inoculum by immobilizing the cells in calcium-alginate beads incorporating cryoprotectants. Appl Environ Microbiol, (56), 3112-3116.
Lengye, Z., & Földényi, R. (2003). Acetochlor as a soil pollutant. Environ Sci Pollut Res, (10), 13-18.
Luo, W., Gu, Q., Chen, W., Zhu, X., Duan, Z., & Yu, X. (2015). Biodegradation of acetochlor by a newly isolated Pseudomonas strain. Appl Biochem Biotechno., (176), 636-644.
Madigan, M.T., & Martinko, J.M. (1997). Microbial growth. In brock biology of microorganisms; Prentice Hall International Inc: New Jersey. 161-172.
Schoebitz, M., Simonin, H., & Poncelet, D. (2012). Starch fi ller and osmoprotectants improve the survival of rhizobacteria in dried alginate beads. J Microencapsul, (29), 532-538.
Souissi, Y., Bourcier, S., Ait-Aissa, S., Maillot- Maréchal, E., Bouchonnet, S., Genty, C., & Sablier M. (2013). Using mass spectrometry to highlight structures of degradation compounds obtained by photolysis of chloroacetamides: case of acetochlor. J Chromatogr A, (1310), 98-112.
Xu, C., Ding, J., Qiu, J., & Ma, Y. (2013). Biodegradation of acetochlor by a newly isolated Achromobacter sp. strain D-12. J Environ Sci Health B, (48), 960-966.
Zheng, J.W., Li, R., Zhu, J.C., Zhang, J., He ,J., Li, S.P., & Jiang, J.D. (2012). Degradation of the chloroacetamide herbicide butachlor by Catellibacterium caeni sp. nov DCA-1T. Int Biodeter Biodegr, (73), 16-22.
Zohar-Perez, C., Ritte, E., Chernin, L., Chet, I., & Nussinovitch, A. (2002). Preservation of chitinolytic pantoae agglomerans in a viable form by cellular dried alginate-based carriers. Biotechnol Prog, (18), 1133-1140.

Các bài báo được đọc nhiều nhất của cùng tác giả

1 2 > >>