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Date Published: 10/10/2020
Online Published: 10/10/2020
Abstract Views: 49
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DOI: https://doi.org/10.52714/dthu.9.5.2020.821
Issue
Vol. 9 No. 5 (2020): Natural Sciences Issue (English)
Section
Natural Sciences Issue (English)
How to Cite
Ha, D. D., Nguyen, T. O., & Ha, H. H. V. (2020). Acetochlor degradation by a mixed culture of P. fluorescens KT3 and B. subtilis 2M6E immobilized in alginate. Dong Thap University Journal of Science, 9(5), 86-92. https://doi.org/10.52714/dthu.9.5.2020.821

Acetochlor degradation by a mixed culture of P. fluorescens KT3 and B. subtilis 2M6E immobilized in alginate

Danh Duc Ha1, , Thi Oanh Nguyen2, Huynh Hong Vu Ha1
1 Department of Engineering and Information Technology, Dong Thap University, Vietnam
2 Center for Chemical Analysis, Dong Thap University, Vietnam

Main Article Content

Abstract

In this study, the acetochlor degradation by two microbial isolates, P. fluorescens KT3 and B. subtilis 2M6E, was determined. The immobilization of the bacterial mixture in alginate beads resulted in higher degradation rates compared to their free cells. The addition of glycerol as a cryoprotectant reducing adverse effects in long-term storage. After storing at 4oC for three months, the cell survivals of free cell with and without the cryoprotectant were 43.0 ± 6.1% and 57.3 ± 5.9%, while data for immobilized bacteria were 64.0 ± 5.3% and 77.6 ± 4.0%, respectively. These results prove that the immobilization of bacteria in alginate and the addition of glycerol can be applied for storing bacteria in a long-term period.

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Keywords

Acetochlor, degradation, P. fluorescens KT3, B. subtilis 2M6E, immobilization

References

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