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Issue: Vol. 15 No. 8 (2026): Natural Sciences Issue (Vietnamese)
Section: Natural Sciences Issue (Vietnamese)
DOI: 10.52714/dthu.ns.2429.1874
Date Published: 28/04/2026
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Nguyen, K. H., & Huynh, T. N. N. (2026). Chemical composition and bioactivity evaluation of essential oil from the leaves of Limnocitrus littoralis (MIQ.) SW. in Phu Yen. Dong Thap University Journal of Science, 15(8), 49-59. https://doi.org/10.52714/dthu.ns.2429.1874

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Chemical composition and bioactivity evaluation of essential oil  from the leaves of Limnocitrus littoralis (MIQ.) SW. in Phu Yen

Nguyen Khanh Hy1, , Huynh Thi Ngoc Ni2
1 Faculty of Natural Sciences, Phu Yen University, Vietnam
2 Faculty of Teacher Education, Ha Tinh University, Vietnam

Main Article Content

Abstract

The leaves of Limnocitrus littoralis (Miq.) Sw. were collected from Phu Yen province. The essential oil from the leaves of Limnocitrus littoralis was obtained by hydrodistillation. The chemical composition of Limnocitrus littoralis essential oil were determined by gas chromatography coupled mass spectrometry (GC-MS) method, with the main constituents including β-Phellandrene (89,8%), β-Myrcene (2,3%), (Z)-β-Ocimene (1,8%), Selin-11-en-4α-ol (0,9%) and α-Pinene (0,7%). The results of the bioactivity test showed that leaf extract essential oil of Limnocitrus littoralis had antibacterial activity against Staphylococcus aureus, Escherichia coli and antifungal activity against Candida albican with IC50 values of 98,17; 89,03 and 15,89 µg/mL, respectively. In addition, the antioxidant activity of the leaf essential oil was higher than vitamin C with IC50 values of 98,17; 89,03 and 15,89  µg/mL, respectively.

Keywords

Antimicrobial, Antioxidant, Essential oil, GC-MS, Limnocitrus littoralis (Miq.) Sw

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Author Biographies

Nguyen Khanh Hy, Faculty of Natural Sciences, Phu Yen University, Vietnam

Lecture, Faculty of Natural Science, Phu Yen University, Viet Nam

Huynh Thi Ngoc Ni, Faculty of Teacher Education, Ha Tinh University, Vietnam

Lecturer, Faculty of Teacher Education, Viet Nam

References

Al-Rowaily, S. L., Abd-ElGawad, A. M., Assaeed, A. M., Elgamal, A. M., Gendy, A. E.-N. G. E., Mohamed, T. A., ... Elshamy, A. I. (2020). Essential oil of Calotropis procera: Comparative chemical profiles, antimicrobial activity, and allelopathic potential on weeds. Molecules, 25(21), 5203. https://doi.org/10.3390/molecules25215203.
Burneo, J. I., Benítez, Á., Calva, J., Velastegui, P., & Morocho, V. (2021). Soil and leaf nutrients drivers on the chemical composition of the essential oil of Siparuna muricata (Ruiz & Pav.) A. DC. from Ecuador. Molecules, 26(10), 2949. https://doi.org/10.3390/molecules 26102949.
Cui, H., Pan, H.-W., Wang, P.-H., Yang, X.-D., Zhai, W.-C., Dong, Y., ... (2018). Essential oils from Carex meyeriana Kunth: Optimization of hydrodistillation extraction by response surface methodology and evaluation of its antioxidant and antimicrobial activities. Industrial Crops and Products, 124, 669–676. https://doi.org/10.1016/j.indcrop.2018.08.041.
Công, X. (Ngày 04 tháng 7 năm 2017). Ngỡ ngàng rừng cam đường dại trĩu quả trên đảo Bé. Dân Trí. Truy cập từ https://dantri.com.vn/du-lich/quang-ngai-ngo-ngang-rung-cam-duong-dai-triu-qua-tren-dao-be-2017070415552284.htm.
Doan, Q. T., Ho, V. D., Le, T. N., Le, T. A., Nguyen, T. H., & Raal, A. (2019). Constituents of essential oils from the leaves of Paramignya trimera (Oliv.) Guillaum from Vietnam. Journal of Essential Oil Bearing Plants, 22(2), 391–395. https://doi.org/10. 1080/0972060X.2019.1582362.
Hadacek, F., & Greger, H. (2000). Testing of antifungal natural products: Methodologies, comparability of results and assay choice. Phytochemical Analysis, 11(3), 137–147. https://doi.org/10.1002/(SICI)1099-1565(200005/06)11:3<137::AID-PCA514>3.0. CO;2-I.
Martins, d. R., , Arantes, M., Candeias, S., Tinoco, F., M. T., Cruz-Morais, J. (2014). Antioxidant, antimicrobial and toxicological properties of Schinus molle L. essential oils. Journal of ethnopharmacology, 151(1), 485-492. https://doi.org/ 10.1016/j.jep.2013.10.063.
Moghaddam, M., Mehdizadeh, L. (2017). Chemistry of essential oils and factors influencing their constituents. In Soft chemistry and food fermentation (pp. 379-419): Elsevier.
Naz, S., Ilyas, S., Parveen, Z., & Javed, S. (2010). Chemical analysis of essential oils from turmeric (Curcuma longa) rhizome through GC-MS. Asian Journal of Chemistry, 22(4), 3153.
Nguyen, T.-T. T., Nguyen, A. V., Diep, T. T., Doan, N. N., Nguyen, T.T.-T. (2022). Essential oil profiles of seeds, peels, and leaves obtained from Limnocitrus littoralis (Miq.) Swingle species, in the Southcentral coast of Vietnam. All Life, 15(1), 908–920. https://doi.org/10.1080/26895293.2022.2112766.
Nguyen, T. L., Doan, V. H., Tran, Q. D., Le, A. T., Raal, A., Usai, D., Sanna, G., Carta, A., Rappelli, P., Diaz, N., Cappuccinelli, P., Zanetti, S., Nguyen, H. T., Donadu, M. G. (2020). Biological activities of essential oils from leaves of Paramignya trimera (Oliv.) Guillaum and Limnocitrus littoralis (Miq.) Swingle. Antibiotics, 9(4), 207. https://doi.org/10.3390/antibiotics9040207.
Phạm, H. H. (2000). Cây cỏ Việt Nam. NXB Trẻ.
Sauvan, N., Renimel, I., Lamy, C., & Dupont, D. (2009). Cosmetic composition containing an extract of Limnocitrus littoralis (U.S. Patent No. 7,527,813 B2).
Sharma, O. P., & Bhat, T. K. (2009). DPPH antioxidant assay revisited. Food Chemistry, 113, 1202–1205. https://doi.org/10.1016/j.foodchem.2008.08.008.
Swingle, W. T. (1940). Limnocitrus, a new genus, also new species of Wenzelia, Paramignya and Atalantia (Rutaceae-Aurantioideae). Journal of the Arnold Arboretum, 21(1), 1–24. https://www.jstor.org/stable/43774260.
Võ, V. C. (2012). Từ điển cây thuốc Việt Nam. Nhà xuất bản Y học.