Investigation on phytochemical composition, antioxidant activity, total phenolic and flavonoid contents of Ludwigia peruviana

Tran Ba Duy1, Pham Ngo Thanh Trung1, Pham Ngo Bao Ngoc1, Nguyen Tu Nguyen1, Duong Quoc An1, Le Minh Nhan2, Nguyen Tram, Thi Thu2,
1 Faculty of Pharmacy, Can Tho University of Medicine and Pharmacy, Can Tho, Viet Nam
2 Faculty of Basic Sciences, Can Tho University of Medicine and Pharmacy, Can Tho, Viet Nam

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Tóm tắt

Ludwigia peruviana, a member of the family Onagraceae, has been reported to possess various biological activities; however, information on its antioxidant properties and phytochemical composition remains limited. This study aimed to investigate the phytochemical constituents and evaluate the antioxidant activity as well as the total phenolic and flavonoid contents of the ethanolic extract of L. peruviana. Aerial parts of L. peruviana were collected in Can Tho City, Viet Nam, between October and November 2024 and extracted by ultrasonic-assisted extraction using 99% ethanol. The phytochemical constituents were determined by employing standard qualitative chemical tests. Antioxidant activity was evaluated using the DPPH radical scavenging assay, while total phenolic content (TPC) and total flavonoid content (TFC) were determined by the Folin–Ciocalteu method and the aluminum chloride colorimetric assay, respectively. This study demonstrated the occurrence of various secondary metabolites, including phenolics, flavonoids, alkaloids, tanins,  fixel oil, carotenoids, saponins and triterpenoids. The ethanolic extract exhibited weak DPPH radical-scavenging activity, with an IC₅₀ value of 229.96 µg/mL. The TPC and TFC were 125.81 ± 7.99 mg GAE/g extract and 39.20 ± 0.49 mg QE/g extract, respectively. To the best of our knowledge, this is the first report on phytochemical composition, DPPH radical scavenging activity and phenolic–flavonoid contents of  L. peruviana. These findings provide a scientific basis for further phytochemical investigations and evaluation of other biological activities of this species.

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Tài liệu tham khảo

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