Bước đầu tinh chế protein tái tổ hợp TRXHA5.1 nguyên liệu sản xuất vaccine phòng virus cúm A/H5N1
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Abstract
Hemagglutinin (HA) is one of the surface antigens of virus H5N1. HA consists of two subunits HA1 and HA2. Since HA1 contains most of the surface antigenic sites of HA, it has been greatly noticed in Avian Influenza vaccine production researches. In this study, to achieve the further aim of creating vaccine against Avian Influenza A/H5N1, we purified recombinant protein - TRXHA5.1 from the observable product of gene trxha5.1 in yeast Pichia pastoris SMD1168 by affinity chromatography. The results showed that initially TRXHA5.1 has been purified successfully.
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References
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[3]. Chen Z, Medical progress, Influenza DNA vaccine: an update, CMJ, Vol.117, No.1 (2004), 125-132.
[4]. Chiu FF et al, Immunological study of HA1 domain of hemagglutinin of influenza H5N1 virut, Biochem. Biophys. Res. Commun. (2009), doi:10.1016/j.bbrc.03.106.
[5]. Daly JM, Mumford JA, Influenza infection. International Veterinary Information Service. Ithaca, New York, USA (2001).
[5]. Horimoto T, Fukuda N, Iwatsuki-Horimoto K, Guan Y, Lim W, Peiris M, Sugii S, Odagiri T, Kawaoka Y, Antigen differences between H5N1 human influenza virutes isolated in 1997 and 2003, J Virol.Vol.66. No.3 (2004), 303-305.
[6]. Mike Brownleade, His-tagged protein purification: Insight into a new kit on the block. www.protein-chem.com/Resources/Proteus%20MC.pdf.
[7]. Natalie BetZ, Purification his-tagged proteins from insect and mammalian cells, Promega corporation. www.promega.com/cnotes/cn009/cn009_06.pdf.
[9]. Steinhauer DA, Role of hemagglutinin cleavage for the pathogenicity of influenza virut,J Virol, Vol.258 (1999), 1-20.
[10]. Terpe K, Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems, Appl. Microbiol. Biotechnol, Vol.60, No.5 (2003), 523–533.
[11]. Webster RG, Influenza: An emerging disease, Emer Infect Dis, Vol.4 (1998), 436-441.
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