Article Sidebar
Views PDF: 17
Metrics
Effects of salinity stress on the germination capacity of xoan orange seeds (Citrus sinensis (L.) cv. Xoan) under conditions in vitro culture
Main Article Content
Abstract
Citrus sinensis (L.) cv. Xoan is one of the delicious orange varieties with high economic value thanks to its sweet taste, and characteristic aroma. It is also a valuable remedy in oriental medicine. Currently, the situation of saltwater intrusion in the Mekong Delta provinces has been complicated, causing heavy damage to crops, including the Xoan orange variety. This study investigated the effects of NaCl at different concentrations on the germination of Citrus seeds under in vitro culture conditions. Seeds are sterilized with 25%; 50% NaClO; and HgCl2 0.1% for 1, 3, 5 minutes and cultured on MS medium, monitoring the survival rate and infection rate during 8 weeks of culture. After determining the disinfection solution with the appropriate concentration and time, the seeds were grown in an environment supplemented with NaCl at a concentration of 2 g/L - 10 g/L and the seed germination rate was monitored during 3 weeks of culture. The results showed that with 0.1% HgCl2 solution in 5 minutes, seeds were best disinfected with a survival rate of 100%. When adding NaCl at different concentrations to the culture medium, the germination rate of Citrus seeds decreased and the seed germination time increased compared to the control treatment. In particular, the NaCl concentration of 10 g/L severely affects the germination of seeds, causing the seeds to show no signs of germination.
Keywords
Orange, Germination, in vitro, Salt stress, Sterilization
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
References
Azevedo, N. A. D., Prico, J. T., Eneas, F. J., Braga, A. C. E., & Gomes, F. E. (2006). Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and saltsensitive maize genotypes. Environmental and experimental botany, 56, 235 - 241.
Chi, T. (March 4, 2024 ). At the peak of saltwater intrusion, 80,000 hectares of rice and fruit trees are at risk of being affected. Tuoi Tre Newspaper. Available https://tuoitre.vn/cao-diem-xam-nhap-man-80-000ha-lua-cay-an-trai-co-nguy-co-bi-anh-huong-20240304093816937.htm
Gamborg, O. L. (2002). Plant tissue culture: Biotechnology Milestones. In Vitro Cellular & Developmental Biology-Plant, 38, 84-92.
Grieve, C.M., S.R. Grattan and E.V. Maas. (2012). Plant salt tolerance. In: W.W. Wallender and K.K. Tanji (eds.). ASCE Manual and Reports on Engineering Practice, 71, 405 - 459.
Hemalatha, G., Renugadevi, J., & Eevera, T. (2017). Studies on seed priming with hydrogen peroxide for mitigating salt stress in rice. International Journal of Current Microbiology and Applied Sciences, 6(6), 691 - 695.
Hernandez, J. A., Campillo, A., Jimenez, A., Alarcon, J. J., & Sevilla, F. (1999). Response of antioxidant systems and leaf water relations to NaCl stress in pea plants. New Phytologist, 141(2), 241 - 251.
Hoang, M. T. (2006). Giao trinh Sinh ly thuc vat [Plant physiology]. Ho Chi Minh City National University Publishing House.
K. V. Weber. (2008). Ecophysiology of high salinity tolerant plants, 1st edn. Springer, Amsterdam.
Le, N. T. N., Tran, T. T., Hoang, T. M. N., Vo, N. T. A., Tran, T. T. L., & Quach, V. T. E. (2022). A study on the effects of iba and ba growth regulators on germination of Tribulus terrestris L. seeds in vitro. Ho Chi Minh City University Of Education Journal Of Science, 19(12), 2076 - 2089.
Munns, R., & James, R. A. (2003). Screening methods for salt tolerance: A case study with tetraploid wheat. Plant and Soil, 253, 201 – 218.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3).
Neljubow, D. N. (1925). Über die Methoden der Bestimmung der Keimfähigkeit ohne Keimprüfung,” Annals D'Essais De Semences, 4(7), 31 – 35.
Nguyen, B. P., Nguyen, Q. S., & Nguyen, B. V. (2021). Morphological Characteristics of Seedless Soan Orange (Citrus sinensis (L.) cv. Soan) Discovered in Hau Giang Province. Vietnam Journal of Agricultural Sciences, 19(12), 1567-1575.
Nguyen, D. C., & To, T. N. L. (2005). Medicinal plants and fruits. Hanoi Medical Publishing House.
Nguyen, T. H. G., Quach, V. C. T., & Nguyen, T. T. (2024). Isolation And Selection Of Bacillus bacteria Antagonizes The Fungus Fusarium sp. Causing Rot Disease On Postharvest Xoan Oranges Fruits In Tra On District, Vinh Long Province. TNU Journal of Science and Technology, 229(09), 101-108.
Nguyen, T. K. L. (Sept. 18, 2022). Vinh Phuc: Applying plant tissue culture to preserve and develop valuable plant genetic resources. Vietnam Science and Technology Magazine. Available https://vjst.vn/vn/tin-tuc/6844/vinh-phuc--ung-dung-nuoi-cay-mo-te-bao-thuc-vat-nham-bao-ton-va-phat-trien-cac-nguon-gen-thuc-vat-co-gia-tri.aspx.
Noreen, Z., & Ashraf, M. (2009). Assessment of variation in antioxidative defense system in salt treated pea (Pisum sativum) cultivars and its putative use as salinity tolerance markers. Journal of plant physiology, 166(16), 1764 - 1774.
Rostovtsev, S. A., & Lyubich, E.S. (1978). Determination of the viability of tree and shrub seeds by staining with indigocarmine in the USSR. Seed science and Technology, 6(3), 869-875.
Shiyab, M. S., Shibli, A. R., & Mohammad, M. M. (2003). Influence of sodium chloride salt stress on growth and nutrient acquisition of sour orange in vitro. Journal of Plant Nutrition, 26, 985–996.
Southern Fruit Tree Institute. (2009). Introducing popular fruit tree varieties in the South. Ho Chi Minh City Agricultural Publishing House.
Thanh, T. (May 3, 2024). Saltwater intrusion in the Mekong Delta is increasingly severe. VnEconomy. Available https://vneconomy.vn/xam-nhap-man-tai-dong-bang-song-cuu-long-ngay-cang-gay-gat.htm
Vu, N. T., Nguyen, N. L., Tran, A. T., Nguyen, N. Q., & Le, T. T. C. (2017). Effect of salinity on germination, growth and yield of two groundnut varieties (L14 and L27). Can Tho University Science Magazine, 53B, 123 – 133.
Xu, S., Hu, B., He, Z., Ma, F., Feng, J., Shen, W., & Yang, J. (2011). Enhancement of salinity tolerance during rice seed germination by presoaking with hemoglobin. International Journal of Molecular Sciences, 12(4), 2488 - 2501.
Yildiz, M., Fatih Ozcan, S., T Kahramanogullari, C., & Tuna, E. (2012). The effect of sodium hypochlorite solutions on the viability and in vitro regeneration capacity of the tissue. The Natural Products Journal, 2(4), 328-331.