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Date Published: 14/06/2021
Online Published: 14/06/2021
Abstract Views: 383
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DOI: https://doi.org/10.52714/dthu.10.3.2021.871
Issue
Vol. 10 No. 3 (2021): Natural Sciences Issue (Vietnamese)
Section
Natural Sciences Issue
How to Cite
Nguyen, T. B. N., Tran, C. L., & Nguyen, T. H. H. (2021). Studying the antioxidant and anti-inflammatory activity of the ethanol extract from the upper parts of Enhydra Fluctuans Lour. in vitro. Dong Thap University Journal of Science, 10(3), 84-92. https://doi.org/10.52714/dthu.10.3.2021.871

Studying the antioxidant and anti-inflammatory activity of the ethanol extract from the upper parts of Enhydra Fluctuans Lour. in vitro

Thi Bich Ngoc Nguyen1, Chi Linh Tran2, Thi Hong Hanh Nguyen3,
1 Student, Faculty of Natural Science Teacher Education, Dong Thap University, Vietnam
2 Student, College of Natural Sciences, Can Tho University, Vietnam
3 Faculty of Natural Science Teacher Education, Dong Thap University, Vietnam

Main Article Content

Abstract

In Vietnam, Enhydra fluctuans Lour (E. fl ucatuans L.) is very popular and considered as a common food. This study was aimed to assess antioxidant and anti-infl ammatory activity in vitro as well as initially identifying the chemical composition of the extract from the upper parts (i.e. stem and leaves) of this vegetable. The antioxidant effi cacy of the ethanol extract was determined based on its ability to neutralize free DPPH, ABTS•+ radicals and the reducing power (RP). The anti-infl ammatory ability of the extract was investigated through the activity of inhibition in protein denaturation. The results showed that the upper parts of E. fl uctuans was able to neutralize free DPPH, ABTS•+ radicals and RP corresponding to IC50 values, respectively 53.36±0.68, 66.36 ±1.47 μg/mL and 74.17±2.27 μg/mL. The compounds of ethanol extract have the anti-infl ammatory activity in vitro of IC50=66.19±3.10 μg/mL. The chemical composition of the upper parts include alkaloids, fl avonoids, steroids, tannins and glycosides. The total fl avonoid and polyphenol contents in the extract from the upper parts were found with the values of 16.73±1.37 mg GAE/g and 138.30±1.89 mg QE/g, respectively. However, saponin compounds were not detected. This shows that the upper parts of E fl uctuans have many potential applications in the fi eld of medicial herbs on antioxidant compounds helping the treatment of diseases caused by oxidative stress and infl ammation.

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Keywords

Enhydra fluctuans Lour., Asteraceae, antioxidant, anti-infl ammatory

References

Arulselvan, P., Fard, M. T., Tan, W. S., Gothai, S., Fakurazi, S., Norhaizan, M. E., & Kumar, S. S. (2016). Role of Antioxidants and Natural Products in Infl ammation. Oxidative Medicine and Cellular Longevity, (2016), 1-15.
Arulselvan, P., Ghofar, H. A. A., Karthivashan, G., Halim, M. F. A., Ghafar, M. S. A., & Fakurazi, S. (2014). Antidiabetic therapeutics from natural source: A systematic review. Biomedicine & Preventive Nutrition, 4(4), 607-617.
Bag, G. C., Devi, P. G., & Bhaigyabati, T. H. (2015). Assessment of total flavonoid content and antioxidant activity of methanolic rhizome extract of three Hedychium species of Manipur valley. International Journal of Pharmaceutical Sciences Review and Research, 30(1), 154-159.
Sagnia, B., Fedeli, D., Casetti, R., Montesano, C., Falcioni, G., & Colizzi, V. (2014). Antioxidant and Anti-Infl ammatory Activities of Extracts from Cassia alata, Eleusine indica, Eremomastax speciosa, Carica papaya and Polyscias fulva. Medicinal Plants Collected in Cameroon, 9(10), 112573.
Elmastas, M., Turkekul, I., Ozturk, L., Gulcin, I., Isildak, O., & Aboul-Enein, H. Y. (2006). Antioxidant activity of two wild edible mushrooms (Morchella vulgaris and Morchella esculanta) from North Turkey. Combinatorial Chemistry & High Throughput Screening, 9(6), 443-448.
Gaestel, M., Kotlyarov, A., & Kracht, M. (2009). Targeting innate immunity protein kinase signalling in inflammation. Nature, 8(6), 480-499.
Ganguly, S. N., Ganguly, T., & Sircar, S. M. (1972). Gibberellins of Enhydra fluctuans. Phytochemistry, 11(12), 3433-3434.
Gothai, S., Arulselvan, P., Tan, W. S., & Fakurazi, S. (2016). Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fi broblasts. Journal of Intercultural Ethnopharmacology, 5(1), 1-6.
Gülçin, İ. (2011). Antioxidant activity of food constituents: an overview. Archives of Toxicology, 86(3), 345-391.
Huỳnh, A. D., Bùi, M. L., & Lâm, T. N. G. (2017a). Khảo sát độc tính cấp và tác dụng bảo vệ gan của cao chiết Rau ngổ (Enhydra fl uctuans Lour., Asteraceae) trên chuột nhắt trắng. Tạp chí Y dược học Cần Thơ, (9), 41-47.
Huỳnh, A. D., Bùi, M. L., & Lâm, T. N. G. (2017b). Khảo sát thành phần hóa học cây Rau ngổ (Enhydra fl uctuans Lour., Asteraceae). Tạp chí Dược học, (492), 53-56.
Joshi, B. S., & Kamat, V. N. (1972). Structure of Enhydrin, a Germacranolide from Enhydra fluctuans Lour. Indian Journal of Chemistry, 10, 771-776.
Kirtikar, K.R., & Basu, B. D. (2002). Indian Medicinal Plants. Delhi: Sri Satguru Publications.
Krishnaswamy, N. R., & Ramji, N. (1995). Sesquiterpene lactones from Enhydra fluctuans. Phytochemistry, 38(2), 433-435.
Krishnaswamy, N. R., & Prasanna, S. (1975). Clerosterol from Enhydra fluctuans. Phytochemistry, 14(7), 1663.
Leelaprakash, G., & Dass, S. M. (2011). Invitro anti-inflammatory activity of methanol extract of Enicostemma axillare. International Journal of Drug Development and Research, 3(3), 189-196.
Ben Salem, M., Affes, H., Athmouni, K., Ksouda, K., Dhouibi, R., Sahnoun, Z., Hammami, S., & Zeghal, K. M. (2017). Chemicals Compositions, Antioxidant and Anti- Infl ammatory Activity of Cynara scolymus Leaves Extracts, and Analysis of Major Bioactive Polyphenols by HPLC. Evidence-Based Complementary and Alternative Medicine, 1-14.
Moreno-Quirós, C. V., Sánchez-Medina, A., Vázquez-Hernández, M., Hernández Reyes, A. G., & García-Rodríguez R. V. (2017). Antioxidant, anti-infl ammatory and antinociceptive potential of Ternstroemia sylvatica. Asian Pacific Journal of Tropical Medicine, 10(11), 1047-1053.
Nathan, C. (2002). Points of control in infl ammation. Nature, 420(6917), 846-852.
Nenadis, N., Wang, L.F., Tsimidou, M., & Zhang, H. (2004). Estimation of scavenging activity of phenolic compounds using the ABTS•+ assay. Journal of Agricultural and Food Chemistry, (52), 4669-4674.
Nguyễn, K. P. P. (2007). Phương pháp cô lập hợp chất hữu cơ. Thành phố Hồ Chí Minh: NXB Đại học Quốc gia Thành phố Hồ Chí Minh, 80-147.
Oyaizu, M. (1986). Studies on product of browning reaction prepared from glucoseamine. The Japanese Journal of Nutrition and Dietetics, 44(6), 307-316.
Piaru, S. P., Mahmud, R., Majid, A. M. S. A., & Nassar, Z. D. M. (2012). Antioxidant and antiangiogenic activities of the essential oils of Myristica fragrans and Morinda citrifolia. Asian Pacific Journal of Tropical Medicine, 5(4), 294-298.
Phạm, H. H. (1999). Cây cỏ Việt Nam 1 (Quyển 3). NXB Trẻ.
Rahman, M. T., Begum, N., Alimuzzaman, M., & Khan, M. O. F. (2002). Analgesic activity of Enhydra fluctuans. Fitoterapia, 73(7-8), 707-709.
Ravipati, A. S., Zhang, L., Koyyalamudi, S. R., Jeong, S. C., Reddy, N., Bartlett, J., ... & Vysetti, B. (2012). Antioxidant and anti-inflammatory activities of selected Chinese medicinal plants and their relation with antioxidant content. BMC Complementary and Alternative Medicine, 12(1), 1-14.
Roginsky, V., & Lissi, E. A. (2005). Review of methods to determine chain-breaking antioxidant activity in food. Food Chemistry, 92(2), 235-254.
Sannigrahi, S., Mazuder, U. K., Pal, D. K., Parida, S., & Jain, S. (2010). Antioxidant Potential of Crude Extract and Different Fractions of Enhydra fl uctuans Lour. Iranian Journal of Pharmaceutical Research, 9(1), 75‐82.
Shah, M., Parveen, Z., & Khan, M. R. (2017). Evaluation of antioxidant, antiinfl ammatory, analgesic and antipyretic activities of the stem bark of Sapindus mukorossi. Complementary and Alternative Medicine, (17), 526.
Sharma, O. P., & Bhat, T. K. (2009). DPPH antioxidant assay revisited. Food chemistry, (113), 1202-1205.
Singleton, V. L., Orthofer, R., & Lamuela- Raventos, R. M. (1999). Analysis of total phenol and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Method Enzymol, (299), 152-178.
Uddin, S. J., Ferdous, M. M., Rouf, R., Alam, M. S., Sarkar, M. A. M., & Shilpi, J. A. (2005). Evaluation of anti-diarrhoeal activity of Enhydra fl uctuans. Journal of Medicine Science, (5), 324-327.
Valko, M., Rhodes, C. J. B., Moncol, J., Izakovic, M. M., & Mazur, M. (2006). Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact, (160), 1-40.
Zhao, Y., Chen, S., Wang, Y., Wang, J., & Lu, J. (2018). Effect of drying processes on prenylflavonoid content and antioxidant activity of Epimedium koreanum Nakai. Journal of Food and Drug Analysis, 26(2), 796-806.

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