Article Sidebar
Section
Mekong Delta Natural Science Conference
How to Cite
To, M. N. T., Nguyen, T. T. N., Trieu, P. H., Nguyen, Q. C. T., & Nguyen, T. T. (2025). Investigation of the chemical composition, antioxidant and antibacterial activities of ethanolic extracts of Ficus hirta var. roxburghii (Miq.) King. Dong Thap University Journal of Science, 14(04S), 189-201. https://doi.org/10.52714/dthu.14.04S.2025.1578
Citation format:
Metrics
Investigation of the chemical composition, antioxidant and antibacterial activities of ethanolic extracts of Ficus hirta var. roxburghii (Miq.) King
Main Article Content
Abstract
The initial study investigated the chemical composition and biological activity of Ficus hirta var. roxburghii (Miq.) King. Quatitative analysis revealed the presence of alkaloids, flavonoids, steroids, triterpenoids, and tannins. Stem extract exhibited high levels of total polyphenols content (39.18 mg GAE/g), flavonoids (73.62 mg QE/g), and tannins (71.86 mg CE/g) compared to leaf extract, correlating with strong antioxidant activity (EC50 values of 192.24 µg/mL for DPPH and 60.73 µg/mL for ABTS•+; Abs0.5 values of 305.24 µg/mL for RP and 226.91 µg/mL for TAC). Conversely, leaf extract contained higher alkaloid content (125.56 mg AE/g) and inhibited Salmonella sp. with a MIC of 625 µg/mL. This differential distribution of bioactive compounds suggests potential for developing specialized natural medicinal products.
Keywords
Kháng khuẩn, kháng oxy hóa, Ngái khỉ, thành phần hóa học
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
References
Abayomi, S. (1993). Medicinal plants and traditional medicine in Africa. Journal of Alternative and Complementary Medicine, 13, 195-238. https://doi.org/10.1016/0378-8741(84)90026-6
Agyare, C., Koffuor, G. A., Boakye, Y. D., & Mensah, K. B. (2013). Antimicrobial and anti-inflammatory properties of Funtumia elastica. Pharmaceutical Biology, 51(4), 418-425. https://doi.org/10.3109/13880209.2012.738330
Chen, C., Peng, X., Chen, J., & Wan, C. (2020). Antioxidant, antifungal activities of ethnobotanical Ficus hirta Vahl. and analysis of main constituents by HPLC-MS. Biomedicines, 8(1), 15. https://doi.org/10.3390/biomedicines8010015
Cheng, J., Yi, X., Wang, Y., Huang, X., & He, X. (2017). Phenolics from the roots of hairy fig (Ficus hirta Vahl.) exert prominent anti-inflammatory activity. Journal of Functional Foods, 31, 79-88. https://doi.org/10.1016/j.jff.2017.01.035
Eng, S. K., Pusparajah, P., Ab Mutalib, N. S., Ser, H. L., Chan, K. G., & Lee, L. H. (2015). Salmonella: a review on pathogenesis, epidemiology and antibiotic resistance. Frontiers in Life Science, 8(3), 284-293. https://doi.org/10.1080/21553769.2015.1051243
Jacoby, G. A., & Archer, G. L. (1991). New mechanisms of bacterial resistance to antimicrobial agents. New England Journal of Medicine, 324(9), 601-612. https://doi.org/10.1056/NEJM199102283240906
Kuete, V., Ngameni, B., Simo, C. F., Tankeu, R. K., Ngadjui, B. T., Meyer, J. J. M., Lall, N. & Kuiate, J. R. (2008). Antimicrobial activity of the crude extracts and compounds from Ficus chlamydocarpa and Ficus cordata (Moraceae). Journal of Ethnopharmacology, 120(1), 17-24. https://doi.org/10.1016/j.jep.2008.07.026
Lobo, V., Patil, A., Phatak, A., & Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4(8), 118. https://doi.org/10.4103/0973-7847.70902
Matić, P., Sabljić, M., & Jakobek, L. (2017). Validation of spectrophotometric methods for the determination of total polyphenol and total flavonoid content. Journal of AOAC International, 100(6), 1795-1803. https://doi.org/10.5740/jaoacint.17-0066
Medini, F., Fellah, H., Ksouri, R., & Abdelly, C. (2014). Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. Journal of Taibah University for Science, 8(3), 216-224. https://doi.org/10.1016/j.jtusci.2014.01.003
Njus, D., Kelley, P. M., Tu, Y. J., & Schlegel, H. B., (2020). Ascorbic acid: The chemistry underlying its antioxidant properties. Free Radical Biology and Medicine, 159, 37-43. https://doi.org/10.1016/j.freeradbiomed.2020.07.013
Pham, H. L. A., He, H., & Pham, H. C. (2008). Free radicals, antioxidants in disease and health. International journal of biomedical science: IJBS, 4(2), 89. https://doi.org/10.4172/0974-8369.1000214
Pham, H. H. (1999). Cây cỏ Việt Nam (Tập 2). Hồ Chí Minh: NXB Trẻ.
Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry, 269(2), 337-341. https://doi.org/10.1006/abio.1999.4019
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
Rizvi, W., Rizvi, M., Kumar, R., Kumar, A., Shukla, I., & Parveen, M. (2010). Antibacterial activity of Ficus lyrata-An in vitro study. Int. J. Pharmacol, 8(2), 7.
Sarker, S. D., Nahar, L., & Kumarasamy, Y. (2007). Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods, 42(4), 321-324. https://doi.org/10.1016/j.ymeth.2007.01.006
Singhal, M., Paul, A., & Singh, H. P. (2014). Synthesis and reducing power assay of methyl semicarbazone derivatives. Journal of Saudi Chemical Society, 18(2), 121-127. https://doi.org/10.1016/j.jscs.2011.06.004
Sirivibulkovit, K., Nouanthavong, S., & Sameenoi, Y. (2018). Based DPPH assay for antioxidant activity analysis. Analytical Sciences, 34(7), 795-800. https://doi.org/10.2116/analsci.18P014
Tabasum, S., Khare, S., & Jain, K. (2016). Spectrophotometric quantification of total phenolic, flavonoid, and alkaloid contents of Abrus precatorius L. seeds. Asian Journal of Pharmaceutical and Clinical Research, 9(2), 371-374.
Tiwari, U., & Cummins, E. (2013). Handbook of plant food phytochemicals: sources, stability and extraction. Wiley-Blackwell, 107-137. https://doi.org/10.1002/9781118464717
Tran, T. G. H., Tran, D. D., & Chu, H. M. (2020). Hoạt tính kháng viêm từ cao chiết lá cây Vú bò (Ficus hirta Vahl) thu hái tại huyện Yên Sơn, tỉnh Tuyên Quang. Tạp chí khoa học Đại học Tân Trào, 6(17), 32-35. https://doi.org/10.51453/2354-1431/2020/384
Wan, C., Chen, C., Li, M., Yang, Y., Chen, M., & Chen, J. (2017). Chemical constituents and antifungal activity of Ficus hirta Vahl. fruits. Plants, 6(4), 44. https://doi.org/10.3390/plants6040044
Wira, D. W., Mardawati, E., Hutauruk, R. O., Bangun, D. E. M., & Kamila, H. E. (2020). Minimum inhibitory concentration of leaf and fruit extract Ficus lyrata Warb against Salmonella thypi bacteria. In IOP Conference Series: Earth and Environmental Science (Vol. 443, No. 1, p. 012046). IOP Publishing. https://doi.org/10.1088/1755-1315/443/1/012046
Xu, D. P., Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., ... & Li, H. B. (2017). Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources. International Journal of Molecular Sciences, 18(1), 96. https://doi.org/10.3390/ijms18010096
Agyare, C., Koffuor, G. A., Boakye, Y. D., & Mensah, K. B. (2013). Antimicrobial and anti-inflammatory properties of Funtumia elastica. Pharmaceutical Biology, 51(4), 418-425. https://doi.org/10.3109/13880209.2012.738330
Chen, C., Peng, X., Chen, J., & Wan, C. (2020). Antioxidant, antifungal activities of ethnobotanical Ficus hirta Vahl. and analysis of main constituents by HPLC-MS. Biomedicines, 8(1), 15. https://doi.org/10.3390/biomedicines8010015
Cheng, J., Yi, X., Wang, Y., Huang, X., & He, X. (2017). Phenolics from the roots of hairy fig (Ficus hirta Vahl.) exert prominent anti-inflammatory activity. Journal of Functional Foods, 31, 79-88. https://doi.org/10.1016/j.jff.2017.01.035
Eng, S. K., Pusparajah, P., Ab Mutalib, N. S., Ser, H. L., Chan, K. G., & Lee, L. H. (2015). Salmonella: a review on pathogenesis, epidemiology and antibiotic resistance. Frontiers in Life Science, 8(3), 284-293. https://doi.org/10.1080/21553769.2015.1051243
Jacoby, G. A., & Archer, G. L. (1991). New mechanisms of bacterial resistance to antimicrobial agents. New England Journal of Medicine, 324(9), 601-612. https://doi.org/10.1056/NEJM199102283240906
Kuete, V., Ngameni, B., Simo, C. F., Tankeu, R. K., Ngadjui, B. T., Meyer, J. J. M., Lall, N. & Kuiate, J. R. (2008). Antimicrobial activity of the crude extracts and compounds from Ficus chlamydocarpa and Ficus cordata (Moraceae). Journal of Ethnopharmacology, 120(1), 17-24. https://doi.org/10.1016/j.jep.2008.07.026
Lobo, V., Patil, A., Phatak, A., & Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4(8), 118. https://doi.org/10.4103/0973-7847.70902
Matić, P., Sabljić, M., & Jakobek, L. (2017). Validation of spectrophotometric methods for the determination of total polyphenol and total flavonoid content. Journal of AOAC International, 100(6), 1795-1803. https://doi.org/10.5740/jaoacint.17-0066
Medini, F., Fellah, H., Ksouri, R., & Abdelly, C. (2014). Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. Journal of Taibah University for Science, 8(3), 216-224. https://doi.org/10.1016/j.jtusci.2014.01.003
Njus, D., Kelley, P. M., Tu, Y. J., & Schlegel, H. B., (2020). Ascorbic acid: The chemistry underlying its antioxidant properties. Free Radical Biology and Medicine, 159, 37-43. https://doi.org/10.1016/j.freeradbiomed.2020.07.013
Pham, H. L. A., He, H., & Pham, H. C. (2008). Free radicals, antioxidants in disease and health. International journal of biomedical science: IJBS, 4(2), 89. https://doi.org/10.4172/0974-8369.1000214
Pham, H. H. (1999). Cây cỏ Việt Nam (Tập 2). Hồ Chí Minh: NXB Trẻ.
Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry, 269(2), 337-341. https://doi.org/10.1006/abio.1999.4019
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
Rizvi, W., Rizvi, M., Kumar, R., Kumar, A., Shukla, I., & Parveen, M. (2010). Antibacterial activity of Ficus lyrata-An in vitro study. Int. J. Pharmacol, 8(2), 7.
Sarker, S. D., Nahar, L., & Kumarasamy, Y. (2007). Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods, 42(4), 321-324. https://doi.org/10.1016/j.ymeth.2007.01.006
Singhal, M., Paul, A., & Singh, H. P. (2014). Synthesis and reducing power assay of methyl semicarbazone derivatives. Journal of Saudi Chemical Society, 18(2), 121-127. https://doi.org/10.1016/j.jscs.2011.06.004
Sirivibulkovit, K., Nouanthavong, S., & Sameenoi, Y. (2018). Based DPPH assay for antioxidant activity analysis. Analytical Sciences, 34(7), 795-800. https://doi.org/10.2116/analsci.18P014
Tabasum, S., Khare, S., & Jain, K. (2016). Spectrophotometric quantification of total phenolic, flavonoid, and alkaloid contents of Abrus precatorius L. seeds. Asian Journal of Pharmaceutical and Clinical Research, 9(2), 371-374.
Tiwari, U., & Cummins, E. (2013). Handbook of plant food phytochemicals: sources, stability and extraction. Wiley-Blackwell, 107-137. https://doi.org/10.1002/9781118464717
Tran, T. G. H., Tran, D. D., & Chu, H. M. (2020). Hoạt tính kháng viêm từ cao chiết lá cây Vú bò (Ficus hirta Vahl) thu hái tại huyện Yên Sơn, tỉnh Tuyên Quang. Tạp chí khoa học Đại học Tân Trào, 6(17), 32-35. https://doi.org/10.51453/2354-1431/2020/384
Wan, C., Chen, C., Li, M., Yang, Y., Chen, M., & Chen, J. (2017). Chemical constituents and antifungal activity of Ficus hirta Vahl. fruits. Plants, 6(4), 44. https://doi.org/10.3390/plants6040044
Wira, D. W., Mardawati, E., Hutauruk, R. O., Bangun, D. E. M., & Kamila, H. E. (2020). Minimum inhibitory concentration of leaf and fruit extract Ficus lyrata Warb against Salmonella thypi bacteria. In IOP Conference Series: Earth and Environmental Science (Vol. 443, No. 1, p. 012046). IOP Publishing. https://doi.org/10.1088/1755-1315/443/1/012046
Xu, D. P., Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., ... & Li, H. B. (2017). Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources. International Journal of Molecular Sciences, 18(1), 96. https://doi.org/10.3390/ijms18010096
Most read articles by the same author(s)
- Thi Bach Le, ThS Thi Kim Phuong Pham, Thi Thanh Xuan Le, PGS Trong Tuan Nguyen, TS Thi Anh Hong Nguyen, Thi Thao Quyen Le, Thi Minh Thu Ho, Thi Bich Tran Nguyen, Toan Duy Ly, Phuoc Duong Pham, Nhat Nghia Cao, Anh Hao Diep, Chemical composition and antifungal activity of Annona reticulata L. , Dong Thap University Journal of Science: Vol. 13 No. 5 (2024): Natural Sciences Issue (English)
- Huynh Vu Thanh Luong, Thanh Phu Le, Quoc Phu Duong, PGS.TS. Trong Tuan Nguyen, TS Duy Toan Pham, Evaluating adsorption of methylene blue using nanocellulose from fresh coconut fiber , Dong Thap University Journal of Science: Vol. 14 No. 04S (2025): Special Issue of Mekong Delta Natural Science Conference