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Date Published: 15/08/2015
Online Published: 15/08/2015
Abstract Views: 43
Views PDF: 23
DOI: https://doi.org/10.52714/dthu.14.8.2015.227
Issue
No. 14 (2015): Part B - Natural Sciences
Section
Natural Sciences Issue (Vietnamese)
How to Cite
Trinh, D. K., & Nguyen, T. H. (2015). Cloning and analyzing the 28s rRNA gene sequence of a cellulase-producing mushroom strain. Dong Thap University Journal of Science, 14, 71-74. https://doi.org/10.52714/dthu.14.8.2015.227

Cloning and analyzing the 28s rRNA gene sequence of a cellulase-producing mushroom strain

Dinh Kha Trinh1, , Thi Huyền Nguyen2
1 PhD student, Thai Nguyen University of Science
2 Thai Nguyen University of Sciences

Main Article Content

Abstract

In this study, we described the results of cloning and analyzing the 28S rRNA gene sequence of a cellulase–producing mushroom strain. The 28S rRNA gene segment was isolated by PCR reaction and cloned into the vector pJET1.2/blunt for nucleotide sequencing. The results showed that the analyzed 28S rRNA gene sequence of the DKVN01 strain is 625 bp and highly homologous to some representatives of the basidiomycetes genus Pleurotus (90.5-100%). Among them, it has the highest homology with that of Pleurotus ostreatus strain (coded AB733315). The DKVN01 is named Pleurotus ostreatus DKVN01, whose sequence of segmenting the 28S rRNA gene has been deposited in GenBank, coded JX987096.

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Keywords

Cellulase, cloning, gene 28S rRNA, Pleurotus ostreatus DKVN01, sequencing.

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