Article Sidebar

PDF
Date Published: 15/08/2015
Online Published: 15/08/2015
Abstract Views: 36
Views PDF: 19
DOI: https://doi.org/10.52714/dthu.14.8.2015.227
Issue
No. 14 (2015): Part B - Natural Sciences
Section
Natural Sciences Issue
How to Cite
Trinh, D. K., & Nguyen, T. H. (2015). Cloning and analyzing the 28s rRNA gene sequence of a cellulase-producing mushroom strain. Dong Thap University Journal of Science, 14, 71-74. https://doi.org/10.52714/dthu.14.8.2015.227

Cloning and analyzing the 28s rRNA gene sequence of a cellulase-producing mushroom strain

Dinh Kha Trinh1,, Thi Huyền Nguyen2
1 PhD student, Thai Nguyen University of Science
2 Thai Nguyen University of Sciences

Main Article Content

Abstract

In this study, we described the results of cloning and analyzing the 28S rRNA gene sequence of a cellulase–producing mushroom strain. The 28S rRNA gene segment was isolated by PCR reaction and cloned into the vector pJET1.2/blunt for nucleotide sequencing. The results showed that the analyzed 28S rRNA gene sequence of the DKVN01 strain is 625 bp and highly homologous to some representatives of the basidiomycetes genus Pleurotus (90.5-100%). Among them, it has the highest homology with that of Pleurotus ostreatus strain (coded AB733315). The DKVN01 is named Pleurotus ostreatus DKVN01, whose sequence of segmenting the 28S rRNA gene has been deposited in GenBank, coded JX987096.

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Keywords

Cellulase, cloning, gene 28S rRNA, Pleurotus ostreatus DKVN01, sequencing.

References

[1]. Bhat M. K. (2000), "Cellulase and related enzymes in biotechnology", Biotechnol. Adv., (18), p. 355-383.
[2]. Dürre P. (1998), "New insights and novel developments in clostridial acetone/butanol/isopropanol fermentation", Appl. Microbiol. Biotechnol., (49), p. 639-648.
[3]. Hennequin C., Abachin E., Symoens F., Lavarde V., Reboux G., Nolard N., Berche P. (1999), "Identification of Fusarium species involved in Human infections by 28S rRNA gene sequencing", J. Clin. Microbiol., (37), p. 3586–3589.
[4]. Hinrikson H. P., Hurst S. F., Lott T. J., Warnock D. W., Morrison C. J. (2005), "Assessment of ribosomal large-subunit D1-D2, internal transcribed spacer 1, and internal transcribed spacer 2 regions as targets for molecular identification of medically important Aspergillus species", J. Clin. Microbiol., (45), p. 2092–2103.
[5]. Trịnh Đình Khá, Quyền Đình Thi, Nguyễn Sỹ Lê Thanh (2007), "Tuyển chọn và nghiên cứu ảnh hưởng của các yếu tố môi trường lên khả năng sinh tổng hợp cellulase của chủng Penicillium sp. DTQ-HK1", Tạp chí Công nghệ Sinh học, (5), tr. 355-362.
[6]. Prasanna D. K., Daisy L. K., David N. F. (2009), "Sequencing and analysis of fungal rRNA operons for development of broad-range fungal PCR assays", Appl. Environ. Microbiol., (75), p. 1559-1565.
[7]. Pham T. H., Quyen D. T., Nghiem N. M. (2010), "Optimization of endoglucanase production by Aspergillus niger VTCC-F021", Aust. J. Basic Appl. Sci., (6), p. 4151-5157.
[8]. Sambrook J., Russell D. W. (2001), Molecular cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
[9]. Saranraj P., Stella D., Reetha D. (2012), "Microbial cellulases and its applications: a review", International Journal of Biochemistry & Biotech Science, (1), p. 1-12.
[10]. Trinh D. K., Quyen D. T., Do T. T., Nguyen T. T. H., Nghiem N. M. (2013), "Optimization of culture conditions and medium components for carboxymethyl cellulase (CMCase) production by a novel basidiomycete strain Peniophora sp. NDVN01", Iranian J. Biotech., (11), p. 251-259.