Ảnh hưởng của loại hóa chất, nồng độ hóa chất và thời gian khử trùng đến kết quả tạo mẫu sạch Sa mộc dầu (Cunninghamia konishii Hayata)

Abstract

Cunninghamia konishii Hayata is a valuable genetic source classified as endangered to be extinct. Thus, it is necessary to use in-vitro propagation for its conservation and development. In this study, its young stems and buds were sterilized as clean samples for in-vitro propagation. The experiments were done repeatedly with NaClO 20%, 30%, 40% for 10-40 minutes; with H₂0₂10%, H₂0₂20%  for 10-40 minutes; with Ca (ClO)₂ 5%, 10%, 15% for 10-20 minutes; and with HgCl₂ 0,1%, 0,15%, 0,2% for 5-15 minutes. The results show that sterilization with HgCl₂ 0.1% for 10 minutes produced the cleanest samples of 73.33%.