Enriching polyphenols from receptaculum Nelumbinis extract using D 101 macroporous
Main Article Content
Abstract
Receptaculum Nelumbinis, often discarded as a by-product after harvesting lotus seeds, actually contains a high concentration of polyphenols, bioactive compounds with significant medicinal value in traditional medicine. To enhance the polyphenol content in concentrated extracts from Receptaculum Nelumbinis, this study has identified the key factors influencing the adsorption and desorption processes using D 101 macroporous adsorption resin. D 101 resin proves to be the most effective material for polyphenol enrichment due to its excellent adsorption properties, cost-effectiveness, and high reusability after desorption, which significantly reduces production costs and minimizes environmental impact. A series of optimization experiments showed that the optimal conditions were a sample solution concentration of 10.000 ppm, 80 % ethanol as the solvent, an adsorption time of 30 minutes, and a desorption duration of 30 minutes using 96 % ethanol. Under these optimized conditions, the use of D 101 resin successfully increased the polyphenol content in the extract to 110,5 ± 0,71 mg GAE/g, demonstrating its efficacy in polyphenol enrichment. These findings pave the way for the application of Receptaculum Nelumbinis extract in pharmaceutical and functional food products, providing a sustainable approach to utilizing this often-discarded by-product and functional food products.
Keywords
D 101, macroporous resin, Eceptaculum nelumbinis, Enrichment, Polyphenols
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
References
Castro-Muñoz, R., Yáñez-Fernández, J., & Fíla, V. (2016). Phenolic compounds recovered from agro-food by-products using membrane technologies: An overview. Food Chemistry, 213, 753-762. https://doi.org/10.1016/j.foodchem.2016.07.030
Conidi, C., Rodriguez-Lopez, A. D., Garcia-Castello, E. M., & Cassano, A. (2015). Purification of artichoke polyphenols by using membrane filtration and polymeric resins. Separation and Purification Technology, 144, 153-161. https://doi.org/10.1016/j.seppur.2015.02.025
Đặng, T. H. N., Trần, Đ. K., Phạm, D. K., Nguyễn, H. T., & Nguyễn, T. H. H. (2023). Khảo sát hoạt tính kháng oxy hóa dịch chiết gương sen. Tạp chí Khoa học Đại học Đồng Tháp, 12(8), 112-120. https://doi.org/10.52714/dthu.12.8.2023.1159
Đỗ, H. B., Đặng, Q. C., Nguyễn, T. D., Đỗ, T. Đ., Phạm, V. H., Vũ, N. L., Phạm, D. M., Phạm, K. M., Đoàn, T. N., Nguyễn, T., & Trần, T. (2006). Cây thuốc và động vật làm thuốc ở Việt Nam (Quyển 2). Hà Nội: NXB Khoa học và Kỹ thuật.
Hu, T., Wang, F., Zhao, Z., Hu, K., & Zhou, C. (2025). Optimization, purification and antioxidant potential of polyphenol ultrasonic-assisted extraction from pecan'Shaoxing'green husk. Food Production, Processing and Nutrition, 7(1), 20. https://doi.org/10.1186/s43014-024-00296-8
Jeon, J.-S., Park, C. L., Syed, A. S., Kim, Y.-M., Cho, I. J., & Kim, C. Y. (2016). Preparative separation of sesamin and sesamolin from defatted sesame meal via centrifugal partition chromatography with consecutive sample injection. Journal of Chromatography B, 1011, 108-113. https://doi.org/10.1016/j.jchromb.2015.12.062
Ping-xiang, W., Ming, W., Kai-li, D., & Feng, N. (2023). Purification of polyphenols from wormwood by macroporous resin and inhibitory activity of its against α-glucosidase. Food and Machinery, 39(7), 13-19. https://www.ifoodmm.cn/journal/vol39/iss7/3
Phạm, H. H. (2003). Cây cỏ Việt Nam: An Illustrated Flora of Vietnam (Quyển 3). Hồ Chí Minh: NXB Trẻ.
Santos, J. H., Almeida, M. R., Martins, C. I., Dias, A. C., Freire, M. G., Coutinho, J. A., & Ventura, S. P. (2018). Separation of phenolic compounds by centrifugal partition chromatography. Green Chemistry, 20(8), 1906-1916. https://doi.org/10.1039/C8GC00179K
Shen, Y., Guan, Y., Song, X., He, J., Xie, Z., Zhang, Y., Zhang, H., & Tang, D. (2019). Polyphenols extract from lotus seedpod (Nelumbo nucifera Gaertn.): Phenolic compositions, antioxidant, and antiproliferative activities. Food science & nutrition, 7(9), 3062-3070. https://doi.org/10.1002/fsn3.1165
Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology, 299, 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
Tungmunnithum, D., Pinthong, D., & Hano, C. (2018). Flavonoids from Nelumbo nucifera Gaertn., a medicinal plant: Uses in traditional medicine, phytochemistry and pharmacological activities. Medicines, 5(4), 127. https://doi.org/10.3390/medicines5040127
Wang, D., Wang, S., Du, Q., Wang, N., Liu, S., Wang, X., & Jiang, J. (2014). Optimization of extraction and enrichment of steroidal alkaloids from bulbs of cultivated Fritillaria cirrhosa. Biomed Res Int, 2014, 258402. https://doi.org/10.1155/2014/258402
Wang, X., Wang, S., Huang, S., Zhang, L., Ge, Z., Sun, L., & Zong, W. (2019). Purification of polyphenols from distiller’s grains by macroporous resin and analysis of the polyphenolic components. Molecules, 24(7), 1284. https://doi.org/10.3390/molecules24071284
Yang, X., Ru, Z., Wang, H., Ben, A., Lin, H., Zhang, X., Li, C., & Yang, L. (2022). Resin adsorption as a means for the enrichment and separation of three terpenoid indole alkaloids: Vindoline, catharanthine and vinblastine from Catharanthus roseus extracts in ionic liquid solution. Industrial Crops and Products, 187, 115351. https://doi.org/10.1016/j.indcrop.2022.115351
Yu, Q., Fan, L., & Li, J. (2020). A novel process for asparagus polyphenols utilization by ultrasound assisted adsorption and desorption using resins. Ultrasonics Sonochemistry, 63, 104920. https://doi.org/10.1016/j.ultsonch.2019.104920
Zhang, Y., Shufen, L., Xiwen, W., & Xing, Z. (2007). Macroporous resin adsorption for purification of flavonoids in Houttuynia cordata Thunb. Chinese Journal of Chemical Engineering, 15(6), 872-876. http://dx.doi.org/10.1016/S1004-9541(08)60017-8
Most read articles by the same author(s)
- Thi Bich Ngoc Nguyen, Chi Linh Tran, Thi Hong Hanh Nguyen, Studying the antioxidant and anti-inflammatory activity of the ethanol extract from the upper parts of Enhydra Fluctuans Lour. in vitro , Dong Thap University Journal of Science: Vol. 10 No. 3 (2021): Natural Sciences Issue (Vietnamese)
- Thi Hong Nhung Dang, Dang Khoa Tran, Duy Khuong Pham, Hong Tham Nguyen, Thi Hong Hanh Nguyen, Investigating antioxidant extraction from Receptaculum Nelumbinis , Dong Thap University Journal of Science: Vol. 12 No. 8 (2023): Natural Sciences Issue (Vietnamese)
- Van Tan Tran, Thi Phuoc An Ngo, Thanh Tuan Tran, Thi Hong Hanh Nguyen, Minh Thao Nguyen, Quoc Tri Tran, Hoang Lin Nguyen, Structures and properties of VB5−/0 clusters from density functional theory calculations , Dong Thap University Journal of Science: Vol. 9 No. 5 (2020): Natural Sciences Issue (English)
- Thi Kiem Ngan Ho, Tran Thien Toan Duong, Thi Lan Huong Nguyen, Thi Hong Hanh Nguyen, Huu Duy Khang Nguyen, Compounds isolated from the chloroform extract of Mahonia Nepalensis DC. trunk , Dong Thap University Journal of Science: Vol. 9 No. 3 (2020): Natural Sciences Issue (Vietnamese)
- Trung Tinh Tran, Chi Bao Mai, Thi Que Tran Tran, Thi Hong Hanh Nguyen, Evaluating the ability to enrich the polyphenol content in the lotus seed epicarp extract by macroporous resin , Dong Thap University Journal of Science: Vol. 15 No. 2 (2026): Natural Sciences Issue (Vietnamese)