Article Sidebar

PDF
Date Published: 10/12/2017
Online Published: 22/06/2023
Abstract Views: 104
Views PDF: 91
DOI: https://doi.org/10.52714/dthu.29.12.2017.537
Issue
No. 29 (2017): Part B - Natural Sciences
Section
Natural Sciences Issue
How to Cite
Nguyen, T. P., Tran, T. Y. N., & Tran, D. G. (2023). Isolating and selecting polyethylene biodegradating bacteria from garbage dumps of Vinh Long Province. Dong Thap University Journal of Science, 29, 92-97. https://doi.org/10.52714/dthu.29.12.2017.537

Isolating and selecting polyethylene biodegradating bacteria from garbage dumps of Vinh Long Province

Thi Pha Nguyen1,, Thi Yen Nhi Tran2, Dinh Gioi Tran3,4
1 Can Tho University
2 Student, Can Tho University
3 Cuu Long Delta Rice Research
4 Cuu Long Delta Rice Research Institute

Main Article Content

Abstract

Applying microbial polyethylene biodegradation is essential to reduce the accumulation of polyethylene pollution. This study has isolated 26 bacteria strains from 5 soil samples of garbage dumps collected from three districts of Vinh Long Province, namely Tra On, Binh Minh and Long Ho. They were isolated on the SM medium supplemented with 20 grams agar and 1 ml hexadecane. On 26 of them, three polyethylene biodegradable bacterial strains (LH4, BM4 and BM5) were the best ones. On both popular polyethylene materials of LDPE and HDPE, BM5 strain worked well. It was then selected to define its taxonomy by sequencing of the 16S rDNA gene region combined with some biochemical reactions, which identified the BM5 bacterial strain belonging to Bacillus genus and closely related with Bacillus amyloliquefaciens species.

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Keywords

Bacillus amyloliquefaciens, HDPE, LDPE, Polyethylene

References

[1]. Arutchelvi, J., M. Sudhakar, A. Arkatkar, M. Doble, S. Bhaduri, D. Ambika, B. Mukesh, U. Sumit and V. Parasu (2008), “Biodegradation of polyethylene and polypropylene”, Indian Journal of Biotechnology, (7), p. 9-22.
[2]. Botre, S., P. Jadhav, L. Saraf, K. Rau and A. Wagle. (2015), “Screening and Isolation of Polyethylene degrading Bacteria from various sources”, International Research Journal of Environment Science, (4), p. 58-61.
[3]. Das, M.P. and S. Kumar (2015), An approach to low-density polyethylene biodegradation by Bacillus amyloliquefaciens”, 3 Biotech, 5 (1), p. 81-86.
[4]. Hadad, D., S. Geresh and A. Sivan (2005), “Biodegradation of polyethylene by the thermophilic bacterium BreviBacillus borstelensis”, Journal of applied microbiology, (98), p. 1093-1100.
[5]. Hussein, A. A., I. K. Al-Mayaly and S. H. Khudeir (2015), “Isolation, Screening and Identification of Low Density Polyethylene (LDPE) degrading bacteria from contaminated soil with plastic wastes”, Mesopotamia Environmental Journal, 1 (4), p. 1-14.
[6]. Ibiene, A. A., H. O. Stanley, and O. M. Immanuel (2013), “Biodegradation of Polyethylene by Bacillus sp. Indigenous to the Niger Delta Mangrove Swamp”, Nig Journal Biotech, (26), p. 68-79.
[7]. Kale, S. K., A. G. Deshmukh, M. S. Dudhare and V. B. Patil (2015), “Microbial degradation of plastic: a review”, J. Biochem Tech, 6 (1), p. 952-961.
[8]. Kavitha R., A. K. Mohanan and V. Bhuvaneswari (2014), “Biodegradation of low density polyethylene by bacteria isolated from oil contaminated soil”, International Journal of Plant, Animal and Environmental Sciences, (4), p. 601-610.
[9]. Nanda S. and S. S. Sahu (2010), “Biodegradability of polyethylene by BreviBacillus, Pseudomonas, and Rhodococcus spp.”, New York Science Journal, (3), p. 95-98.
[10]. Roberts M. S., L. K. Nakamura, and F. M. Cohan (1996), “Bacillus vallismortis sp. nov., a Close Relative of Bacillus subtilis, Isolated from Soil in Death Valley, California”, International Journal of Systematic Bacteriology, 46 (2), p. 470-475.