Isolating and selecting polyethylene biodegradating bacteria from garbage dumps of Vinh Long Province

Thi Pha Nguyen1,, Thi Yen Nhi Tran2, Dinh Gioi Tran3,4
1 Can Tho University
2 Student, Can Tho University
3 Cuu Long Delta Rice Research
4 Cuu Long Delta Rice Research Institute

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Applying microbial polyethylene biodegradation is essential to reduce the accumulation of polyethylene pollution. This study has isolated 26 bacteria strains from 5 soil samples of garbage dumps collected from three districts of Vinh Long Province, namely Tra On, Binh Minh and Long Ho. They were isolated on the SM medium supplemented with 20 grams agar and 1 ml hexadecane. On 26 of them, three polyethylene biodegradable bacterial strains (LH4, BM4 and BM5) were the best ones. On both popular polyethylene materials of LDPE and HDPE, BM5 strain worked well. It was then selected to define its taxonomy by sequencing of the 16S rDNA gene region combined with some biochemical reactions, which identified the BM5 bacterial strain belonging to Bacillus genus and closely related with Bacillus amyloliquefaciens species.

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