Pharmacognosy, phytochemical study and in vitro antioxidant activity of butterfly pea flowers (Clitoria ternatea L., Fabaceae) in Bac Lieu
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Abstract
The study aims to determine the plant characteristics, preliminary chemical composition and in vitro antioxidant activity of ethanol 96% and aqueous extract of the flowers of Butterfly pea. By analyzing the specimen and consulting with the botanist, it was identified that the Butterfly pea was collected in Bac Lieu wasClitoria ternatea L, Fabaceae. In addition, qualitative results have shown that in the flowers of the Butterfly pea there is the presence of chemical compounds: Anthocyanine, flavonoids, carotenoids, essential oils, free triterpenoids, tannins, saponins, organic acids, reducing agents and polyuronid compounds. In addition, the in vitro antioxidant capacity was performed by the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity in comparison with vitamin C as standard. The test sample was a 96% ethanol extract and water extract of the Butterfly pea flowers showed IC50 values of 362.94 μg/mL and 297.39 μg/mL, respectively, although lower than the standard vitamin C (IC50 = 4.72 μg/mL). In conclusion, the aqueous extract showed better antioxidant activity than ethanol 96% extract. The results proved that the Butterfly pea flowers had the potentialbiological activityto provide the basis for further applicants.
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Keywords
Butterfly pea flower, antioxidant activity, pharmacognosy, DPPH assay
References
Kamkaen, N., & Wilkinson, J. M. (2009). The antioxidant activity of Clitoria ternatea flower petal extracts and eye gel. Phytotherapy Research, 23 (11), 1624-1625.
Kumar S., & Bhat K. (2011), In-vitro cytotoxic activity studies of Clitoria ternatea linn flower extracts, Int J. Pharma Sci. Rev Res., 6, 120-121.
Phrueksanan, W., Yibchok-anun, S., & Adisakwattana, S.(2014). Protection of Clitoria ternatea flower petal extract against free radical-induced hemolysis and oxidative damage in canine erythrocytes. Research in veterinary science, 97(2), 357-363.
Võ, V. C. (2012). Từ điển cây thuốc Việt Nam, NXB Y học. Tập 1, 883-885.
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