Article Sidebar

PDF
Date Published: 14/04/2022
Online Published: 14/04/2022
Abstract Views: 167
Views PDF: 125
DOI: https://doi.org/10.52714/dthu.11.2.2022.941%20
Issue
Vol. 11 No. 2 (2022): Natural Sciences Issue (Vietnamese)
Section
Natural Sciences Issue (Vietnamese)
How to Cite
Dang, T. P. T., Ngo, M. T., Lam, N. K. T., Tran, T. M. T., Nguyen, B. T., & Nguyen, T. P. (2022). Effects of ethyl methane sulfonate on the mutagenesis of gloxinia (Sinningia speciose (G. Lodd.) Hiern) under in vitro conditions. Dong Thap University Journal of Science, 11(2), 74-79. https://doi.org/10.52714/dthu.11.2.2022.941

Effects of ethyl methane sulfonate on the mutagenesis of gloxinia (Sinningia speciose (G. Lodd.) Hiern) under in vitro conditions

Thi Phuong Thao Dang1, Minh Tri Ngo1, Ngoc Kim Truc Lam1, Thi My Tien Tran1, Buu Thuan Nguyen1, Thi Pha Nguyen1,
1 Biotechnology Research and Development Institute, Can Tho University, Vietnam

Main Article Content

Abstract

This study was carried out to evaluate the effect of Ethyl methane sulfonate on mutant formation in gloxinia (Sinningia speciose (G. Lodd.) Hiern). Stem segments with axillary buds of in vitro gloxinia samples were treated with six different EMS concentrations (0%, 0.2%, 0.4%; 0.6%; 0.8%; 1%) for 1 hour and 2 hours. The results showed that, the higher EMS concentration, longer treatment time, the lower the survival rate, shoot regeneration rate and the more different morphologies appear compared to the control. In which, EMS concentration of 0.8% for 1 hour and EMS concentration of 0.2% for 2 hours treatments showed a different phenotype of the seedlings compared with the control, and had a high regeneration ability. The ITS regions of these two treatments were sequenced to detect 13 SNPs (Single nucleotide polymorphism), in which EMS treatment at a concentration of 0.8% for 1 hour recorded 12 SNP markers, and 0.2% for 2 hours recorded 1 NSP marker.

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Keywords

EMS, mutant, Sinningia speciosa (G. Lodd.) Hiern, sequencing ITS

References

Altindal, D., & Altindal, N. (2018). Effect of Ethyl Methanesulphonate (EMS) applications on in vitro growth of sunflower (Helianthus annuus L. cv. Palancı-I) under salinity conditions. Journal of the Institute of Science and Technology, 8(4): 351-359.
Amini, M. (2014). Ethyl Methanesulfonate. In Philip Wexler (Editor), Encyclopedia of Toxicology, (522-524). Academic Press.
Devi, A. S., & Mullainathan, L. (2011). Genotoxicity effect of ethyl methanesulfonate on root tip cells of chilli (Capsicum annuum L.). World Journal of Agricultural Sciences, 7(4), 368-374.
Jabeen, N., & Mirza, B. (2004). Ethyl methanesulfonate induces morphological mutations in Capsicum annuum. International journal of agriculture & biology, 6(2), 340-345.
Nguyễn, Q. T., Vũ, N. L., Nguyễn, X. T., Nguyễn, C. H., & Nguyễn, T. L. A. (2004). Nghiên cứu nhân nhanh cây hoa chuông (Sinningia speciosa). Tạp chí Khoa học kỹ thuật Nông nghiệp, 4, 239-244.
Rogers, S. O., & Bendich, A. J. (1988). Extraction of DNA from plant tissues. Plant Molecular Biology Manual, A6, 1-10.
Sarmiento, F. B., Leigh, J. A., & Whitman, W. B. (2011). Chapter three-Genetic Systems for Hydrogenotrophic Methanogens. Methods in Enzymology, 494, 43-73.
Senapati, S. K., & Rout, G. R. (2008). In vitro mutagenesis of rose with ethylmethanesulphonate (EMS) and early selection using RAPD markers. Advances in Horticultural Science, 22(3), 218-222.
Trần, N. D. (2011). Sổ tay thực hành Sinh học phân tử. Nhà xuất bản Đại học Cần Thơ. 169 trang.
Vũ, H. H., & Nguyễn, T. L. A. (2013). Ảnh hưởng của xử lý đột biến in vitro bằng Ethyl Methane Sulphonate (EMS) kết hợp chiếu xạ tia gamma đến sự biến dị ở cây hoa cẩm chướng ((Dianthus caryophyllus L.). Tạp chí Khoa học và Phát triển, 11(8), 1092-1100.
White, T. J., Bruns, T., Lee, S. J. W. T., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA Genes for phylogenetics. Academic Press, 38, 317.
Zaitlin, D. (2012). Intraspecific diversity in Sinningia speciosa (Gesneriaceae: Sinningieae), and possible origins of the cultivated florist’s gloxinia. AoB Plants, pls039, 1-17.