Isolating and selecting thermotolerant lactic acid bacteria from agricultural wastes

Hoang Dang Long Bui1, Xuan Phong Huynh, Ngoc Thanh Nguyen, Thi Phuong Dung Ngo
1 Can Tho University

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Abstract

This study investigates strains of lactic acid bacteria with good thermotolerant anti-bacterial and fermentation abilities from agricultural wastes collected from Mekong Delta areas and Binh Duong province. The results are 36 lactic acid bacteria strains were isolated with corresponding biochemical properties. Among them, 8 strains could grow at high temperature up to 47°C. And 29 strains could inhibit the indicated strain of Bacillus subtilis. Especially, 5 strains (ND3, ND1, ND4, CT1, BA4) could produce inhibition zones larger than 10 mm. Among 10 strains analyzed for acid fermentation, strain CC2 could ferment sucrose 4% (w/v) medium and produce highest total acid concentration at 38C of 1.425 g/L after 5 days.

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