Ethanol production from cocoa pod hydrolysate by Saccharomyces cerevisiae
Main Article Content
Abstract
Cocoa pod (with more than 40% cellucose) is one of agro-waste sources in agro-production and can be used for bioethanol production. The purpose of this study is to investigate the ability of ethanol production from cocoa pod hydrolysate and identify fermentation conditions by means of Saccharomyces cerevisiae. The research results show that the Saccharomyces cerevisiae fermentation VVD3 obtained 5.14% (v/v) ethanol and the sugar consumption efficiency was 97.66%, in the identified conditions as follows: the initial density at 106 cells/ml, 8.62% (w/v) reducing sugar and pH 5.5 at 30-32oC during 7 days of fermentation. This research reveals the potential of bioethanol production by using agro-waste sources of cocoa pod.
Article Details
![Creative Commons License](http://i.creativecommons.org/l/by-nc/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Keywords
cellulose, cocoa pod hydrolysate, ethanol fermentation, Saccharomyces cerevisiae
References
[2]. D. A. Costa, C. J. de Souza, P. S. Costa, M. Q. Rodrigues, A. F. dos Santos, M. R. Lopes, H. L. Genier, W. B. Silveira, and L. G. Fietto (2014), “Physiological characterization of thermotolerant yeast for cellulosic ethanol production”, Applied Microbiology and Biotechnology, (98), p. 3829-3840.
[3]. Lương Đức Phẩm (2009), Nấm men công nghiệp, NXB Khoa học và Kỹ thuật, Hà Nội.
[4]. Ngô Thị Phương Dung, Lý Huỳnh Liên Hương và Huỳnh Xuân Phong (2011), “Phân lập, tuyển chọn nấm men và xác định điều kiện ảnh hưởng quy trình lên men rượu vang dưa hấu”, Tạp chí Khoa học Trường Đại học Cần Thơ, (số 18b), tr. 137-145.
[5]. I. Orlandi, R. Ronzulli, N. Casatta, and M. Vai (2013), “Ethanol and acetate acting as carbon/ energy sources negatively affect yeast chronological aging”, Oxidative Medicine and Cellular Longevity, 2013 (ID 802870), p. 1-10.
[6]. M. E. Pampulha and M. C. Loureiro-Dias (1989), “Combined effect of acetic acid, pH and ethanol on intracellular pH of fermenting yeast”, Applied Microbiology and Biotechnology, 31 (5), p. 547-550.
[7]. Phạm Thiếu Quân, Lê Thị Vân An, Phan Lê Bảo Ngọc, Trần Hải My, Nguyễn Ngọc Thạnh và Huỳnh Xuân Phong (2013), Nghiên cứu khả năng thủy phân và điều kiện lên men sản xuất ethanol sinh học từ vỏ trái ca cao, Đề tài nghiên cứu khoa học cấp cơ sở, Trường Đại học Cần Thơ, Cần Thơ.
[8]. O. A. Samah, S. Sias, Y. G. Hua, and N. N. Hussin (2011), “Production of ethanol from cocoa pod hydrolysate”, ITB Journal of Science, 43 (2), p. 87-94.
[9]. R. E. H. Sims, W. Mabee, J. N. Saddler, and M. Taylor (2010), “An overview of second generation biofuel technologies”, Bioresource Technology, (101), p. 1570-1580.
[10]. K. Tasun, P. Chose, and K. Ghen (1970), “Sugar determination of DNS method”, Biotechnology and Bioengineering, (12), p. 921.
Most read articles by the same author(s)
- Ngoc Tram Anh Lu, Hoang Dang Long Bui, Tri Trung Dang, Ngoc Thanh Nguyen, Hoang Dang Long Bui, Thi Phuong Dung Ngo, Xuan Phong Huynh, Isolation and selection of lactic acid bacteria applied in papaya juice fermentation , Dong Thap University Journal of Science: No. 21 (2016): Part B - Natural Sciences
- Hoang Dang Long Bui, Xuan Phong Huynh, Ngoc Thanh Nguyen, Thi Phuong Dung Ngo, Isolating and selecting thermotolerant lactic acid bacteria from agricultural wastes , Dong Thap University Journal of Science: No. 31 (2018): Part B - Natural Sciences
- Ngoc Thanh Nguyen, Thi Anh Tho Pham, Minh Chau Luu, Hoang Dang Long Bui, Xuan Phong Huynh, Evaluation of culture conditions for producing pigments and monacolin K from Monascus purpureus , Dong Thap University Journal of Science: Vol. 11 No. 2 (2022): Natural Sciences Issue (Vietnamese)
- Xuan Phong Huynh, Thieu Quan Pham, Ngoc Thanh Nguyen, Thi Phuong Dung Ngo, A study on the hydrolysis possibility of cocoa pod via ethanol fermentation , Dong Thap University Journal of Science: No. 16 (2015): Part B - Natural Sciences