Isolation and selection of lactic acid bacteria applied in papaya juice fermentation
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Abstract
This study is to isolate and to select lactic acid bacteria possibly applied in papaya juice fermentation. Seventeen lactic acid bacterial isolates were purified from 7 natural fermented papaya juice samples. Of which, five isolates (DD21, DD41, DD42, DD52 and DD61) can grow well on MRS agar containing 2.0% (v/v) lactic acid. The testing result of papaya juice fermentation at 37ºC in 48 hours showed that DD52 is a good strain in term of acid production and growth (with 8.48 g/L of total acid and 5.78 x 108 CFU/mL of cell concentration) and selected to apply in papaya juice fermentation. Strain DD52 was identified asLactobacillus plantarum with 99% of identity.
Article Details
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Keywords
lactic acid bacteria, papaya, probiotic, Lactobacillus plantarum
References
[2]. F. Guarner and G. J. Schaafsma (1998), “Probiotics”, International Journal of Food Microbiology, (39), p. 237-238.
[3]. W. H. Lin, C. F. Hwang, L. W. Chen, and H. Y. Tsen (2006), “Viable counts characteristic evalu- ation for commercial lactic acid bacteria products”, Food Microbiology, 23 (1), p. 74-81.
[4]. P. Marteau, M. de Vrese, C. J. Cellier, and J. Schrezenmier (2001), “Protection from gasteroin- testinal diseases with the use of probiotics”, American Journal Clinical Nutrition, 73 (2), p. 430S-436S.
[5]. T. Mattila-Sandholm, P. Myllarinen, R. Crittenden, G. Mogensen, R. Fonden, and M. Saarela (2002), “Technological challenges for future probiotic foods”, International Dairy Journal, (12), p. 173-182.
[6]. E. B. Minelli and A. Benini (2008), “Relationship between number of bacteria and their probiotic effects”, Microbial Ecology in Health and Disease, (20), p. 180-183.
[7]. Huỳnh Xuân Phong, Dương Kim Mỹ, Nguyễn Thị Pha Ly, Nguyễn Ngọc Thạnh, Trần Vũ Phương và Ngô Thị Phương Dung (2008), Sản xuất nước cà chua lên men bằng vi khuẩn lactic, Đề tài nghiên cứu khoa học cấp cơ sở (mã số: T2007-04), Trường Đại học Cần Thơ.
[8]. N. P. Shah (2000), “Probiotic bacteria: Selective enumeration and survival in dairy food”, Jounal of Dairy Science, 83 (4), p. 894-907.
[9]. Subhashini (2014), “Bioprospecting of lactic acid bateria for potentiality as probiotics”, International Journal of Microbiological Research, 5 (2), p. 90-97.
[10]. Nguyễn Ngọc Thạnh, Huỳnh Xuân Phong, Nguyễn Thị Việt Trinh, Huỳnh Thị Thu Ba, Bùi Hoàng Đăng Long, Ngô Thị Phương Dung (2015), “Phân lập và tuyển chọn vi khuẩn lactic ứng dụng trong lên men sữa chua bổ sung tảo Spirulina”, Tạp chí Khoa học Đại học Cần Thơ, 40 (1), tr. 8-14.
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